![]() ![]() ![]() Quickly, so as to minimize the possible time for contamination of the specimen in the source tube or flask, the person would use the inoculating loop with their writing hand to retrieve the specimen, and then sterilize the top of the tube or flask again before immediately closing it.The person would then open the tube or flask containing the specimen source and briefly hold the top of it in the flame, to kill unwanted microbes. Preparing to execute the specimen transfer, the person would hold both of the tubes or flasks in one hand, probably the opposite of the writing hand.The person would not allow the loop to touch anything except the specimen itself, until the entire procedure is finished. The person would start the fire, and move the end of the inoculating loop, in a slow back-and-forth motion, through the top of the blue part of the flame.A person would assemble the closed tube or flask from which-and the closed tube or flask to which-the specimen is to be transferred, an inoculating loop, and a fire source, all on a clean, preferably microbe-free surface with some overhead protection from airborne microbes.Such a procedure, using a flame sterilization method, might occur as follows: It is used whenever specimens are to be transferred between media, for example, when subculturing. fomites), and contamination of the specimen they are working on, which is especially important when a pure culture is desired. Medical or clean asepsis reduces the number of organisms and prevents their spread surgical or sterile asepsis includes procedures to eliminate micro-organisms from an area and is practised by nurses in operating theatres and treatment areas.Īseptic technique is the name given to the procedures used by microbiologists to prevent microbial contamination of themselves, which may result in infection, contamination of the environment they are working in (e.g. (2000) suggest that there are two types of asepsis: medical and surgical asepsis. ![]() This can be achieved by ensuring that only sterile equipment and fluids are used during invasive medical and nursing procedures. It is a method used to prevent contamination of wounds and other susceptible sites by organisms that could cause infection. The founder of the technique is considered to be Joseph Lister. This includes medical techniques and laboratory techniques, such as with microbiological cultures.Īseptic technique is the effort taken to keep patients as free from hospital micro-organisms as possible (Crow 1989). List of terms related to Aseptic techniqueĪseptic technique refers to a procedure that is performed under sterile conditions. Risk calculators and risk factors for Aseptic techniqueĬauses & Risk Factors for Aseptic technique ![]() US National Guidelines Clearinghouse on Aseptic techniqueĭirections to Hospitals Treating Aseptic technique Ongoing Trials on Aseptic technique at Clinical Ĭlinical Trials on Aseptic technique at Google Most recent articles on Aseptic techniqueĪrticles on Aseptic technique in N Eng J Med, Lancet, BMJĬochrane Collaboration on Aseptic technique ![]()
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